sacas9 grna Search Results


sacas9 transgene  (Addgene inc)
90
Addgene inc sacas9 transgene
A, Schematic of gRNA design to target R176Q mutant Ryr2 allele in genomic DNA of R176Q/+ mouse. The mutant codon resulting in the R176Q mutation (red) and the silent Rsr restriction site (green) are marked in the sequence. The guide sequence (22-nt, blue) pairs with the DNA target marked as blue bar upstream of a 5’-NNGRRT adjacent motif (PAM; orange). Cas9 will mediate a double-stranded break ~3 bp upstream of the PAM (red triangle). B, Schematic representation of the <t>SaCas9</t> AAV9 constructs. Original backbone with BbsI cloning sites is used as control.
Sacas9 Transgene, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sacas9+grna/pmc06206886-112-16-20?v=Addgene+inc
Average 90 stars, based on 1 article reviews
sacas9 transgene - by Bioz Stars, 2026-08
90/100 stars
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1313 paav u6 bbsi mlui grna sa hlp sacas9 ha ollas spa  (Addgene inc)
93
Addgene inc 1313 paav u6 bbsi mlui grna sa hlp sacas9 ha ollas spa
A, Schematic of gRNA design to target R176Q mutant Ryr2 allele in genomic DNA of R176Q/+ mouse. The mutant codon resulting in the R176Q mutation (red) and the silent Rsr restriction site (green) are marked in the sequence. The guide sequence (22-nt, blue) pairs with the DNA target marked as blue bar upstream of a 5’-NNGRRT adjacent motif (PAM; orange). Cas9 will mediate a double-stranded break ~3 bp upstream of the PAM (red triangle). B, Schematic representation of the <t>SaCas9</t> AAV9 constructs. Original backbone with BbsI cloning sites is used as control.
1313 Paav U6 Bbsi Mlui Grna Sa Hlp Sacas9 Ha Ollas Spa, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sacas9+grna/pmc12767720-1187-0-7?v=Addgene+inc
Average 93 stars, based on 1 article reviews
1313 paav u6 bbsi mlui grna sa hlp sacas9 ha ollas spa - by Bioz Stars, 2026-08
93/100 stars
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1x107 hek293ft cells  (Addgene inc)
90
Addgene inc 1x107 hek293ft cells
A, Schematic of gRNA design to target R176Q mutant Ryr2 allele in genomic DNA of R176Q/+ mouse. The mutant codon resulting in the R176Q mutation (red) and the silent Rsr restriction site (green) are marked in the sequence. The guide sequence (22-nt, blue) pairs with the DNA target marked as blue bar upstream of a 5’-NNGRRT adjacent motif (PAM; orange). Cas9 will mediate a double-stranded break ~3 bp upstream of the PAM (red triangle). B, Schematic representation of the <t>SaCas9</t> AAV9 constructs. Original backbone with BbsI cloning sites is used as control.
1x107 Hek293ft Cells, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sacas9+grna/pmc09934614__media___1-13-6-22?v=Addgene+inc
Average 90 stars, based on 1 article reviews
1x107 hek293ft cells - by Bioz Stars, 2026-08
90/100 stars
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aav9camkiiα egfp 2a sacas9 u6 grna ptk2b  (Sangon Biotech)
86
Sangon Biotech aav9camkiiα egfp 2a sacas9 u6 grna ptk2b
A, Schematic of gRNA design to target R176Q mutant Ryr2 allele in genomic DNA of R176Q/+ mouse. The mutant codon resulting in the R176Q mutation (red) and the silent Rsr restriction site (green) are marked in the sequence. The guide sequence (22-nt, blue) pairs with the DNA target marked as blue bar upstream of a 5’-NNGRRT adjacent motif (PAM; orange). Cas9 will mediate a double-stranded break ~3 bp upstream of the PAM (red triangle). B, Schematic representation of the <t>SaCas9</t> AAV9 constructs. Original backbone with BbsI cloning sites is used as control.
Aav9camkiiα Egfp 2a Sacas9 U6 Grna Ptk2b, supplied by Sangon Biotech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sacas9+grna/pm41690512-53-7-10?v=Sangon+Biotech
Average 86 stars, based on 1 article reviews
aav9camkiiα egfp 2a sacas9 u6 grna ptk2b - by Bioz Stars, 2026-08
86/100 stars
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kz801: pmagic (l1-r5) hu6::sacas9 grna scaffold; ef1a promoter (plasmid #121814)  (Addgene inc)
N/A
Standard format: Plasmid sent in bacteria as agar stab
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1518_paav-u6-sa-egfp-grna-hlp-sacas9-ha-ollas-spa (plasmid #109316)  (Addgene inc)
N/A
Standard format: Plasmid sent in bacteria as agar stab
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ji702: pmagic (l5-l4) hu6::sacas9 grna scaffold; rip (plasmid #121820)  (Addgene inc)
N/A
Standard format: Plasmid sent in bacteria as agar stab
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kb701: pmagic (l5-l4) hu6::sacas9 grna scaffold; cmv promoter (plasmid #121818)  (Addgene inc)
N/A
Standard format: Plasmid sent in bacteria as agar stab
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ji501: pmagic (l3-l2) 3x ha epitope tag + polya; hu6::sacas9 grna scaffold (plasmid #121841)  (Addgene inc)
N/A
Standard format: Plasmid sent in bacteria as agar stab
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1313_paav-u6-sa-bbsi-mlui-grna-hlp-sacas9-ha-ollas-spa (plasmid #121482)  (Addgene inc)
N/A
Standard format: Plasmid sent in bacteria as agar stab
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lf901: pmagic (l1-r5) mu6::sacas9 grna scaffold (plasmid #121811)  (Addgene inc)
N/A
Standard format: Plasmid sent in bacteria as agar stab
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ji601: pmagic (l1-r5) hu6::sacas9 grna scaffold; rip (plasmid #121815)  (Addgene inc)
N/A
Standard format: Plasmid sent in bacteria as agar stab
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Image Search Results


A, Schematic of gRNA design to target R176Q mutant Ryr2 allele in genomic DNA of R176Q/+ mouse. The mutant codon resulting in the R176Q mutation (red) and the silent Rsr restriction site (green) are marked in the sequence. The guide sequence (22-nt, blue) pairs with the DNA target marked as blue bar upstream of a 5’-NNGRRT adjacent motif (PAM; orange). Cas9 will mediate a double-stranded break ~3 bp upstream of the PAM (red triangle). B, Schematic representation of the SaCas9 AAV9 constructs. Original backbone with BbsI cloning sites is used as control.

Journal: Circulation research

Article Title: In Vivo Ryr 2 Editing Corrects Catecholaminergic Polymorphic Ventricular Tachycardia

doi: 10.1161/CIRCRESAHA.118.313369

Figure Lengend Snippet: A, Schematic of gRNA design to target R176Q mutant Ryr2 allele in genomic DNA of R176Q/+ mouse. The mutant codon resulting in the R176Q mutation (red) and the silent Rsr restriction site (green) are marked in the sequence. The guide sequence (22-nt, blue) pairs with the DNA target marked as blue bar upstream of a 5’-NNGRRT adjacent motif (PAM; orange). Cas9 will mediate a double-stranded break ~3 bp upstream of the PAM (red triangle). B, Schematic representation of the SaCas9 AAV9 constructs. Original backbone with BbsI cloning sites is used as control.

Article Snippet: The AAV-CRISPR vector, 1255_pAAV-U6-SA-BbsI-MluI-gRNA-CB-SACas9-HA-OLLAS-spA was generated by the Lagor lab (Addgene plasmid 109320) based on the SaCas9 transgene from px602, Addgene plasmid 61593 (gift of Dr. Feng Zhang).

Techniques: Mutagenesis, Sequencing, Construct, Cloning, Control

A, Experimental timeline of AAV9 injections and subsequent phenotypical analysis B, Representative examples of simultaneous recording of surface ECG lead I (L-1) and intracardiac ventricular electrogram showing sustained polymorphic ventricular tachycardia in a R176Q/+ mouse treated with placebo (AAV9-Bbsl-SaCas9; left) and normal sinus rhythm in an AAV9-gRNA-SaCas9 treated R176Q/+ mouse (right). C, Bar graph summarizing the incidence of inducible sustained VT in R176Q/+ mice and WT littermates treated with AAV9-Bbsl-SaCas9 or AAV9-gRNA-SaCas9, respectively. Abbreviations: P, postnatal day; SC, subcutaneous. *p<0.05.

Journal: Circulation research

Article Title: In Vivo Ryr 2 Editing Corrects Catecholaminergic Polymorphic Ventricular Tachycardia

doi: 10.1161/CIRCRESAHA.118.313369

Figure Lengend Snippet: A, Experimental timeline of AAV9 injections and subsequent phenotypical analysis B, Representative examples of simultaneous recording of surface ECG lead I (L-1) and intracardiac ventricular electrogram showing sustained polymorphic ventricular tachycardia in a R176Q/+ mouse treated with placebo (AAV9-Bbsl-SaCas9; left) and normal sinus rhythm in an AAV9-gRNA-SaCas9 treated R176Q/+ mouse (right). C, Bar graph summarizing the incidence of inducible sustained VT in R176Q/+ mice and WT littermates treated with AAV9-Bbsl-SaCas9 or AAV9-gRNA-SaCas9, respectively. Abbreviations: P, postnatal day; SC, subcutaneous. *p<0.05.

Article Snippet: The AAV-CRISPR vector, 1255_pAAV-U6-SA-BbsI-MluI-gRNA-CB-SACas9-HA-OLLAS-spA was generated by the Lagor lab (Addgene plasmid 109320) based on the SaCas9 transgene from px602, Addgene plasmid 61593 (gift of Dr. Feng Zhang).

Techniques:

A, Diagram showing where PCR primers bind to sequences in exons 3-6 upstream of mutation R176Q in exon 8 (green) and primers targeting exons 11-13 downstream of R176Q (orange). B, Levels of RyR2 mRNA in cardiac tissue from R176Q/+ mice or WT littermates, treated with BbsI-SaCas9 or gRNA-SaCas9. C, Western blot analysis of RyR2 and JPH2 protein levels in heart tissue from R176Q/+ mice or WT littermates. D, Corresponding quantifications of RyR2, and E, JPH2 proteins levels normalized to GAPDH. *p<0.05.

Journal: Circulation research

Article Title: In Vivo Ryr 2 Editing Corrects Catecholaminergic Polymorphic Ventricular Tachycardia

doi: 10.1161/CIRCRESAHA.118.313369

Figure Lengend Snippet: A, Diagram showing where PCR primers bind to sequences in exons 3-6 upstream of mutation R176Q in exon 8 (green) and primers targeting exons 11-13 downstream of R176Q (orange). B, Levels of RyR2 mRNA in cardiac tissue from R176Q/+ mice or WT littermates, treated with BbsI-SaCas9 or gRNA-SaCas9. C, Western blot analysis of RyR2 and JPH2 protein levels in heart tissue from R176Q/+ mice or WT littermates. D, Corresponding quantifications of RyR2, and E, JPH2 proteins levels normalized to GAPDH. *p<0.05.

Article Snippet: The AAV-CRISPR vector, 1255_pAAV-U6-SA-BbsI-MluI-gRNA-CB-SACas9-HA-OLLAS-spA was generated by the Lagor lab (Addgene plasmid 109320) based on the SaCas9 transgene from px602, Addgene plasmid 61593 (gift of Dr. Feng Zhang).

Techniques: Mutagenesis, Western Blot

A, Schematic picture showing gRNA-SaCas9 and “reporter” AAV9 co-injected in R176Q/+ mice. The “reporter” virus with cloned targeted sequence was cut by gRNA-SaCas9 but not the control BbsI-SaCas9. The mCherry protein is only expressed in cardiomycytes with SaCas9 expression. B, Representative confocal images of ventricle myocytes from R176Q/+ mice co-injected with Bbsl-SaCas9 (control) + “reporter” and gRNA-SaCas9 (editing) + “reporter”, merged with bright field (BF) and red fluorescence (RFP) signal overlay. Scale bar, 100 μm. C, Bar graph showing percentages of mCherry-positive cardiomyocytes. D-F, Summary data for Ca2+ spark frequency, SR Ca2+ load, and SR Ca2+ transient amplitude. *p<0.05, **p<0.01, ***p<0.001.

Journal: Circulation research

Article Title: In Vivo Ryr 2 Editing Corrects Catecholaminergic Polymorphic Ventricular Tachycardia

doi: 10.1161/CIRCRESAHA.118.313369

Figure Lengend Snippet: A, Schematic picture showing gRNA-SaCas9 and “reporter” AAV9 co-injected in R176Q/+ mice. The “reporter” virus with cloned targeted sequence was cut by gRNA-SaCas9 but not the control BbsI-SaCas9. The mCherry protein is only expressed in cardiomycytes with SaCas9 expression. B, Representative confocal images of ventricle myocytes from R176Q/+ mice co-injected with Bbsl-SaCas9 (control) + “reporter” and gRNA-SaCas9 (editing) + “reporter”, merged with bright field (BF) and red fluorescence (RFP) signal overlay. Scale bar, 100 μm. C, Bar graph showing percentages of mCherry-positive cardiomyocytes. D-F, Summary data for Ca2+ spark frequency, SR Ca2+ load, and SR Ca2+ transient amplitude. *p<0.05, **p<0.01, ***p<0.001.

Article Snippet: The AAV-CRISPR vector, 1255_pAAV-U6-SA-BbsI-MluI-gRNA-CB-SACas9-HA-OLLAS-spA was generated by the Lagor lab (Addgene plasmid 109320) based on the SaCas9 transgene from px602, Addgene plasmid 61593 (gift of Dr. Feng Zhang).

Techniques: Injection, Virus, Clone Assay, Sequencing, Control, Expressing, Fluorescence